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Western immunoblots of U87 cells treated with specific GRM3 agonist, LY379268 (Ag, 1 μM), riluzole (RIL, (1 μM), agonist plus GRM3 specific antagonist, LY341495 (Ant, (1 μM), or Ag plus RIL. Cells were pre-incubated with antagonist or RIL for 24 and 48 hours prior to agonist challenge. The membranes were probed with pERK antibody and GAPDH antibody as loading controls.
