Skip to main content
. 2019 Mar 21;12:655–665. doi: 10.2147/IDR.S194462

Figure 1.

Figure 1

The gene of PA0847 affects biofilm formation and motility phenotypes in Pseudomonas aeruginosa. Notes: (A) Swimming motility of P. aeruginosa PAO1 (WT), the PA0847 mutants and the strains complemented with empty vector or PA0847, colony morphologies taken after 20–24 hours of incubation at 37°C, and swimming motility assays performed on medium supplemented with 0.3% agar. (B) Swarming motility of P. aeruginosa PAO1 (WT), the PA0847 mutants and the strains complemented with empty vector or PA0847, colony morphologies taken after 36–48 hours of incubation at 37°C, and swarming motility assays performed on medium supplemented with 0.45% agar. (C) Statistical analysis of biofilms of P. aeruginosa PAO1, the PA0847 mutant or the strains complemented with empty vector or PA0847. The biofilm data shown are mean ± SD of at least three replicates. PAO1: P. aeruginosa PAO1 WT; ΔPA0847-1 or ΔPA0847-2: two PA0847 transposon mutant strains; PAO1/pACYC184: PAO1 harboring the empty vector pACYC184; ΔPA0847/pACYC184: ΔPA0847-1 or ΔPA0847-2 harboring empty vector pACYC184; ΔPA0847-1/PA0847 or ΔPA0847-2/PA0847: ΔPA0847-1 or ΔPA0847-2 mutant strains harboring full-length PA0847 with its native promoter and RBS cloned into pACYC184.

Abbreviation: WT, wild type.