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. 2019 Mar 21;12:655–665. doi: 10.2147/IDR.S194462

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© 2019 Zhang et al.

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Diguanylate cyclase activity of PA0847 GGDEF domain. Notes: (A) Molecular architecture of PA0847. Domains are predicted by using the Conserved-Domain Database search from NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) and transmembrane helices are predicted in the TMHMM Server (http://www.cbs.dtu.dk/services/TMHMM/). The amino acid number of each domain is indicated above the corresponding bar. (B) DGC activity of PA0847 GGDEF domain is detected by HPLC assays. GTP and c-di-GMP standard HPLC profiles are indicated above the assays. (C) The binding of GTPγS to PAS-GGDEF domain showed a binding affinity (K_d) of 15.3±1.3 µM. (D) Gel filtration profiles of PAS-GGDEF and GGDEF. The calculated MW of each peak based on protein standards is indicated above the peak. (E) Congo Red staining of wild-type PAO1 and ΔPA0847.