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. 2001 Dec 18;98(26):15014–15019. doi: 10.1073/pnas.261455698

Figure 3.

Figure 3

Expression of photoreceptor-specific genes in RPE cultures under ngn2 induction. (A, C, E, and F) In situ hybridization of E6 RPE culture infected with RCAS-ngn2 with digoxigenin-labeled antisense RNA probes against visinin (A), IRBP (C), the red pigment gene (E), and α-phosphodiesterase (PDE) (F). (B and D) In situ hybridization of cultured E6 RPE cells infected with RCAS-GFP with antisense RNA probes against visinin (B) and IRBP (D). (G) Immunostaining of RPE culture infected with RACS-ngn2 with anti-Visinin protein. (H) A Visinin+ cell with cone photoreceptor morphology. (I) Double-labeling for IRBP mRNA (blue/purple) and Visinin protein (red). Arrows point to double-labeled cells. Magnification: A–G, ×50; H and I, ×200.