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. 2001 Dec 18;98(26):15014–15019. doi: 10.1073/pnas.261455698

Figure 4.

Figure 4

Expression of ganglion cell markers in RPE cultures under ngn2 induction. (A) Immunocytochemistry with RA4. (B) Immunocytochemistry with anti-Calretinin. (C) In situ hybridization with digoxigenin-labeled RNA probes for cNSCL1. (D) Higher magnification of RA4+ cells from ngn2-expressing RPE culture. (E) Double-labeling with RA4 (in red) and anti-Visinin Ab (in green). Arrow points to an RA4+/Visinin− cell in a cluster of Visinin+ cells. (F–H) Double-labeling with RA4 (in red, F) and anti-Calretinin Ab (in green, G). H is a merge of F and G. Magnification: A–C, ×50; D and E, ×100; F–H, ×80.