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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Cancer Res. 2019 Jan 25;17(5):1024–1035. doi: 10.1158/1541-7786.MCR-18-0117

Figure 2. Exploring the PCG2 tumor transcriptome.

Figure 2.

(A) Hierarchal clustering analysis of RNA-Seq data shows top 100 most differentially expressed genes from tumor versus unaffected tibialis anterior muscle in PCG2 mice (n=3). (A’) RPKM values show induction of Hh targets, ES-related genes, and select developmental genes in PCG2 tumors versus TA muscle. (B) Immuno-phenotype indicates PCG2 tumors, but not PtchlaZ eRMS, robustly express ES markers Nkx2.2 and Cd99, as well as methyltransferase EZH2. (C) TEM on ultra-thin PCG2 tumor sections. (D) GSEA shows enrichment of indicated gene sets. Scale bars are 50 μm.