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Luciferase reporter assay of transactivation activity for wild‐type and mutant forms of hepatocyte nuclear factor 4α (HNF4α). The luciferase/β‐galactosidase (Luc/β‐gal) activity ratios are shown as mean ± standard error of the mean from three independent experiments. *P < 0.05 (Student's t‐test). The cell lysates were subjected to immunoblot analysis with anti‐HNF4α antibody (sc‐374229; Santa Cruz Biotechnology, Santa Cruz, CA, USA), **P < 0.01
