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. 2018 Oct 13;10(3):602–612. doi: 10.1111/jdi.12931

Figure 7.

Figure 7

Activation of nuclear factor (erythroid‐derived 2)‐related factor 2 (Nrf2) by docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in immortalized mouse Schwann (IMS32) cells. (a) IMS32 cells were cotransfected with a reporter plasmid (pNL[NlucP/ARE/Hygro]) and a control plasmid (pGL4.53[luc2/PGK]). After transfection, IMS32 cells were cultured with 7.5 μmol/L DHA or 25 μmol/L EPA for 12 h. Each value represents the mean ± standard error of three experiments. **P < 0.01 compared with the bovine serum albumin (BSA) control. IMS32 cells were cultured with (b,c) 7.5 μmol/L DHA or (d,e) 25 μmol/L EPA for the indicated times. Nuclear and cytosolic proteins were used for western blotting analyses. Each value represents the mean ± standard error of three experiments. *P < 0.05, **P < 0.01 compared with each BSA control. ARE, anti‐oxidant response element.