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. 2019 May 2;9:6834. doi: 10.1038/s41598-019-43274-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential abundance of OTUs with significant response to stressors using DESeq2 and the generalized linear model framework. Only OTUs present in greater than 15 samples were included in the analysis. Each point represents a single OTU that increased or decreased significantly (FDR corrected p < 0.05) with the stressor or stressor interaction. Each row and dot color corresponds to a microbial family (i.e. multiple OTUs from a single family are increased in multiple treatments). Family name in [] represents a recommended taxonomic annotation by GreenGenes. Box colors represent the stressor type: main effect = yellow, two-way interaction = purple, three-way interaction = red. The colored line within each box represents the mean log2FoldChange for OTUs with significant changes in that stressor type. The dashed colored line within comparisons represents the mean log2FoldChange for OTUs with significant changes within the individual stressor effects and interactions. The gray colored line at 0 log2FoldChange denotes no effect or no interaction.