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. 2019 May 2;9:6830. doi: 10.1038/s41598-019-43366-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RSM result in a 3D surface (a) and a contour (b). The biomass response from the interaction of fructose concentration and yeast extract concentration in g/L each. The peak of the surface is the optimal point, which is located at fructose 72.2 g/L, yeast extract 21.5 g/L, with a biomass response of 46.1 g/L.