Fig. 6.

TMEM33 overexpression induces cytotoxicity in a PC2-dependent manner. a Cell viability determined by measuring the number of Cherry positive cells using a cell sorter. CD8ER (red dots) was used as a negative control. Cells were transfected with TMEM33 (black dots) or PC2 alone (red dots), or with the mix TMEM33/PC2 (black dots). The relative (to the CD8ER conditions) number of viable cells expressing Cherry is illustrated. b Relative number of DAPI positive cells. One TMEM33 complemented TMEM33^−/− data point (4.66) is out of scale. c Relative number of annexin positive cells. d Relative caspase 3/7 activity. e LDH release in either a conditional PCT cell line expressing CD8ER (in red) or TMEM33 (in black), without (in gray) or with DOX induction (in black) at 48 and 96 h. Cells were treated with vehicle (DMSO) or TM (1 μg/ml) for 16 h. f Same for caspase 3/7 activity. g Effect of the cathepsins inhibitor leupeptin (LEUP; 25 μM) on LDH release in a conditional PCT cell line expressing TMEM33 (black dots) or CD8ER (red dots) without or with DOX induction (48 h), as indicated. Values are means ± SEM overlaid with dot plots. One star indicates p < 0.05, two stars p < 0.01 and three stars p < 0.001, with a one-way permutation test used to evaluate statistical significance. Source data are provided as a Source Data file