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. 2019 May 2;9:6806. doi: 10.1038/s41598-019-43340-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Direct delivery of molecules into NIH-3T3 cells. (a–d) Schematics of Au nanostraw membrane stamping into cells with different nanostraw heights of 0 (a), 1.3 (b), 2.4 (c), and 5.0 µm (d). (e–h) Optical and fluorescence images after the calcein and PI delivery using 0- (e), 1.3- (f), 2.4- (g), and 5.0-µm-high nanostraws (h). Total cell number N = 224 in (e), 476 in (f), 535 in (g) and 494 in (h). (i) Summarized data of nanostraw size and density, molecular delivery, and viability.