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. 2019 May 2;6:46. doi: 10.1038/s41597-019-0053-2

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© The Author(s) 2019

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Fig. 3 — Example of the plate layout of run 2 of the type 1 experiment for the Batch 2 items (experiment 11). Here only ITEM12 to ITEM32 have technical replicates on the plate. For runs 1 and 3 (i.e. biological replicates) of the same type 1 experiment, the wells in columns 9 to 12 were treated with other ITEMS to have at least one technical replicate per ITEM. CaCl₂ and Valinomycin were added as reference chemicals and are both diluted from starting concentrations of 11 μM and 165 nM respectively; i.e. are diluted following the 1:2 scheme like the MNMs or added manually at a fixed concentration on each plate.