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. 2019 May 2;9:6843. doi: 10.1038/s41598-019-43139-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IgG seroreactivity of immuno-dominant OMPs at different vaccination time points. Reactivity of immunogenic proteins to pre- (T0 or N) and post-vaccination (T12 or T20) sera from 25 human vaccinees and 10 mice (T6) are quantified and represented as a heat map. The colour blocks represent the fluorescence signal intensity for reactivity against total IgG antibodies in serum. Antigens are ranked according to magnitude of difference between human T20 and T0. PilQ_25–777 represents the full length protein while PilQ_25–580 is missing the C-domain. Proteins are represented as the last 4 digits of their gene numbers (NMBH4476_), letters represent the oligomeric state (M, monomer; D, dimer; T, trimer; Te, tetramer; O, octamer; De, decamer). *NMB 1994 (NadA) is not expressed in strain H44/76 and was obtained from MC58 strain. More details on the individual proteins are given in Supplementary Table S2.