Table 2.

Major IgG reactive antigens to human and mouse antisera following administration of MenPF-1 OMV vaccine^a.

	NMBH4476b	Folded	iOMP?	Relative abundance in OMVc	IgG reactivityd	p-valuee
Human
PorA	0791	YES	YES	12,840	166 ± 27	5.93 × 10−7
RmpM	0378	YES	NO	1,970	149 ± 21	3.01 × 10−8
PorB	1982	YES	YES	13,870	114 ± 21	2.00 × 10−6
PilQ25–777	1758	YES	YES	850	68 ± 9	2.73 × 10−9
OpcA (Df)	1117	YES	YES	1,230	87 ± 26	1.42 × 10−3
OpcA (Mf)	1117	YES	YES	1,230	68 ± 20	1.94 × 10−3
BamC	1243	YES	NO	340	53 ± 10	7.00 × 10−6
PilQ25–580	1758	YES	YES	850	29 ± 5	7.00 × 10−3
GNA1162 (M)	1046	YES	NO	180	14.8 ± 4.5	1.6 × 10−2
GNA1162 (D)	1046	YES	NO	180	14.1 ± 3.8	4.82 × 10−3
Mouse
PorA	0791	YES	YES	12,840	509 ± 46	2.00 × 10−6
RmpM	0378	YES	NO	1,970	434 ± 17	2.75 × 10−8
LysM/M23	0741	YES	NO	NDg	187 ± 33	3.54 × 10−4
PilQ25–580	1758	YES	YES	850	106 ± 16	1.92 × 10−4
PilQ25–777	1758	YES	YES	850	114 ± 17	1.53 × 10−4

^aMajor reactive antigens are defined as p < 0.05.

^bProteins are represented as the last 4 digits of their NCBI protein accession number or gene numbers (NMBH4476_).

^cValue represents the relative protein abundance in OMV as estimated by mass spectrometry.

^dDifference in total IgG sero-reactivity between human pre-vaccination (T0) and post-3-vaccination (T20) sera or mouse naïve (N) and post-2-vaccination (T6) sera. The reactivity is given as arbitrary fluorescence measurement ± standard error.

^eIgG reactivities at 0, 12 and 20 weeks were analysed for differences using one-way repeated measures ANOVA with Greenhouse-Greisser correction. For the mouse sera, p-values were determined by two-tailed T-test.

^fM, monomer; D, dimer or multimer.

^gNot detected.