Fig. 5.

Heatmap of differentially expressed genes and GSEA of diagnosis versus relapse NPM1^mut loss samples. a Differentially expressed genes (significant at 0.05 level of the univariate test) from RNA-Seq were assessed using the ClassComparison option from BRB-ArrayTools. Blue indicates upregulation and red downregulation between groups. b Diagnosis samples show enrichment of MYC_UP.V1_UP and BIOCARTA_CELLCYCLE_PATHWAY gene sets. c Relapse samples were enriched in KEGG_MAPK_SIGNALING_PATHWAY, WNT_UP.V1_UP gene sets and genes which are downregulated in NPM1^mut samples (VERHAAK_AML_WITH_NPM1_MUTATED_DN). ES, enrichment score; FDR, false discovery rate; NES, normalized enrichment score