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. 2019 May 2;10:2063. doi: 10.1038/s41467-019-10026-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Cytosine methylation decreases DNA-binding affinity of REF6-ZnF in vitro. EMSA with NAC004 (AT1G02230) and AT4G11710 probes. REF6-ZnF specifically bound the unmethylated probes, but had significantly lower (or no) affinity for probe sequences containing one or more methylated cytosines. Source data are provided as a Source Data file