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. 2019 Apr 26;7:62. doi: 10.3389/fcell.2019.00062

FIGURE 1.

FIGURE 1

Effects of Environmental enrichment (EE) and Social enrichment (SE) on the number and morphological maturation of newborn dentate granule cells (DGCs). (A) Experimental design. (B) Representative images of Doublecortin-expressing (DCX+) cells in control housing (CH), EE, and SE animals. (C) Number of DCX+ cells. (D) Percentage of “Horizontal type” and “Vertical type” DCX+ cells in the different experimental conditions. (E) Representative images of newborn dentate granule cells (DGCs) transduced with either Cerulean-, mCherry-, or Venus-encoding retroviruses in the different experimental conditions. (F) Total dendritic length of newborn DGCs transduced with either Cerulean-, mCherry-, or Venus-encoding retroviruses in the different experimental conditions. (G) Sholl’s analysis of dendritic branching in Cerulean-transduced newborn DGCs. (H) Sholl’s analysis of dendritic branching in mCherry-transduced newborn DGCs. (I) Sholl’s analysis of dendritic branching in Venus-transduced newborn DGCs. ML, molecular layer; GCL, granule cell layer; H, hilus. Green scale bar: 250 μm. White scale bar: 50 μm. Yellow scale bar: 10 μm. Blue triangles: “Horizontal type” DCX+ cells. Yellow triangles: “Vertical type” DCX+ cells. ∗∗0.01 > p ≥ 0.001; ∗∗∗p < 0.001. Asterisks indicate changes with respect to CH animals.