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. 2019 Apr 26;10:900. doi: 10.3389/fimmu.2019.00900

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Copyright © 2019 Heigl, Singh, Saez-Gimenez, Kaes, Van Herck, Sacreas, Beeckmans, Vanstapel, Verleden, Van Raemdonck, Verleden, Vanaudenaerde, Hartl and Vos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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G-MDSCs isolated from lung transplant patients functionally supress T cell proliferation. The suppressive effect of CD66b⁺-MACS-isolated MDSCs (isolated from lung transplant recipients; 1 with CLAD and 1 stable) on CFSE labeled T cell CD4⁺ (green) and CD8⁺ (purple) proliferation. (A) Different ratios of MDSC vs. T cells (1:6 and 1:2) were assessed and compared with T cell proliferation without MDSCs. P0 represents undivided cells, P1 cells divided 1 time; P2 cells divided twice and so on. T cell proliferation ratio is portion of divided cells over all cells. The bar graphs represent the proliferation index compared to control conditions (n = 2).