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. 2019 Apr 26;10:913. doi: 10.3389/fimmu.2019.00913

Figure 2.

Figure 2

IL-12 and IL-2 similarly expand predominant clones of HMBPP-activated Vγ2Vδ2 T cells, but the IL-12-induced expansion does not require endogenous IL-2. (A–D) A comparison of the percentage of CDR3 length (A,B) and analysis of VDJ sequences (C,D) in Vγ2- and Vδ2-bearing TCR expressed by cells expanded by HMBPP + IL-12 and HMBPP + IL-2, respectively. RT-PCR was used to specifically amplify the CDR3 regions of Vγ2- and Vδ2-bearing TCR cDNA. (E) Endogenous IL-2 is not involved in IL-12 induced expansion of HMBPP-activated Vγ2Vδ2 T cells. The bar graph shows that anti-IL-2 neutralizing mAbs from two sources (αIL-2-BD and αIL-2-RD) fail to block the ability of IL-12 to expand HMBPP-activated Vγ2Vδ2 T cells (left panel), but these anti-IL-2 mAbs not isotype controls (ISO-BD/ISO-RD) significantly reduced the IL-2 expansion of HMBPP-activated Vγ2Vδ2 T cells in the HMBPP + IL-2 culture. PBMC were co-cultured with HMBPP (10 ng/ml) plus IL-2 (5 ng/ml) or IL-12 (25 ng/ml) in the presence or absence of 5 ug/ml anti-IL-2/IL-12 antibody or isotype controls for 7 days. Data shown as mean ± SEM of four independent experiments pooled from 20 healthy controls, ****p < 0.0001, t-test.