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. 2019 Apr 26;10:913. doi: 10.3389/fimmu.2019.00913

Figure 5.

Figure 5

Vγ2Vδ2 T cells expanded by HMBPP + IL-12 exhibit central/effector memory phenotypes and maintain tissue trafficking markers. (A) Shown in the left are representative flow-cytometry quadrats displaying memory surrogate markers of Vγ2Vδ2 T cells based on CD27 and CD45RA expression in HMBPP + IL-12 cultures at day 0 and 7. Data at day 0 are similar to those in the medium only control culture. Shown in the right are representative flow cytometry histograms uncovering the phenotype of the αβ T cells that are cultured and gated in cytometry similarly to Vγ2Vδ2 T cells. (B) Bar graph shows that Vγ2Vδ2 T cells expanded by HMBPP + IL-12 express effector and central memory phenotypes. (C) Flow histogram and graph data show that expanded Vγ2+Vδ2+ T cells maintain expression of tissue-trafficking/residence markers CCR5 and LAF-1. Flowcytometry data are gated on live individual lymphocytes, CD3+, then Vγ2Vδ2+, and then representative markers. Data shown are mean ± SEM of three independent experiments pooled from 15 healthy controls.