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. 2019 Apr 26;9:126. doi: 10.3389/fcimb.2019.00126

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Copyright © 2019 Liu, Lei and Kadowaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lotmaria passim clone expressing tdTomato. Differential interference contrast (DIC), red fluorescence (tdTomato), and the merged (Merge) images of wild type L. passim (A–C) and L. passim stably transfected with pTrex-Neo-tdTomato. The images of G418-resistant single clone just after the expansion (0 week, D–F), cultured for 14 weeks using the modified FP-FB medium with (14 weeks +G418, G–I) or without (14 weeks -G418, J–L) G418 are shown. White bar = 30 μm.