Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 26;9:126. doi: 10.3389/fcimb.2019.00126

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Liu, Lei and Kadowaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Efficiency of transient transfection of L. passim by electroporation. Wild type L. passim (A), L. passim stably transfected with pTrex-Neo-tdTomato (B), and the mixture of A and B (C) were analyzed for red fluorescent intensity using FACS. L. passim was electroporated with pTrex-Neo-tdTomato at different voltages (D–F: 1.2 kV, G–I: 1.5 kV, J–L: 1.8 kV) followed by culture in the modified FP-FB medium for 48 h. Thirty thousand cells were then analyzed by FACS as above and the experiments were repeated three times. Fraction of tdTomato-expressing parasites is shown as %.