Figure 3.

Cas9 protein expression and growth of Cas9-expressing L. passim clone. (A) The cell lysates of wild type (WT) and FLAG-tagged Cas9 expressing (Cas9) L. passim were analyzed by western blot using anti-FLAG antibody (upper panel). The same SDS-PAGE gel was stained by Coomassie Brilliant Blue as the loading control (lower panel). The size (kDa) of protein molecular weight marker (MW) is at the left. (B) Growth of WT (black) and Cas9 (red) L. passim in the modified FP-FB medium. The experiments were repeated three times and the mean value with error bar (± SD) is indicated for each time point. There is no statistical difference between WT and Cas9 except day 1 (P < 0.015) by unpaired t-test (two-tail).