Figure 4.

Sensitivity of PCR product amplified with genomic DNA of L. passim expressing Cas9 and LpMT sgRNA against T7 endonuclease I. Genomic DNA was extracted from wild type L. passim and the pooled parasites expressing Cas9 and LpMT sgRNA (Cas9+LpMT sgRNA) and used as the template to amplify LpMT genomic DNA fragment containing the sgRNA targeted site by PCR. The PCR products treated with (+) or without (–) T7 endonuclease I (T7EI) were analyzed by 2% agarose gel. The positions of 500 and 1,000 bp bands in molecular weight marker (MW) are shown at the right.