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. 2019 Apr 8;11:2769–2781. doi: 10.2147/CMAR.S194073

Figure 4.

Figure 4

MTDH is a direct target gene of miR-877 in CRC cells. (A) A sequence complementary to that of miR-877 was discovered in the 3′-UTR of MTDH. The mutant miR-877-binding sequence is also shown. (B) miR-877 mimics or miR-NC and pGL3-WT-MTDH-3′-UTR or pGL3-MUT-MTDH-3′-UTR were co-transfected into SW480 and HCT116 cells. After 48 h of transfection, luciferase reporter assay was performed to detect luciferase activity. *P<0.05 versus miR-NC. (C) MTDH mRNA expression in 53 pairs of CRC tissues and adjacent non-tumor tissues was detected through RT-qPCR. *P<0.05 versus non-tumor tissues. (D and E) Western blotting analysis was carried out to measure MTDH protein expression in several pairs of CRC tissues and adjacent non-tumor tissues. N: non-tumor tissues; T: CRC tissues. *P<0.05 versus non-tumor tissues. (F) Spearman’s correlation analysis was applied to evaluate the relationship between miR-877 and MTDH mRNA levels in CRC tissues. r=−0.5381, P<0.0001. (G and H) SW480 and HCT116 cells were transfected with miR-877 or miR-NC. Results of RT-qPCR and western blotting analyses indicated that miR-877 upregulation decreased MTDH mRNA and protein expression. *P<0.05 versus miR-NC.