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. 2019 Apr 8;11:2769–2781. doi: 10.2147/CMAR.S194073

Figure 6.

Figure 6

Restoration of MTDH expression abolishes the phenotypic changes caused by miR-877 overexpression in CRC cells. miR-877 mimics were co-transfected with MTDH overexpression plasmid (pc-MTDH) or empty pcDNA3.1 plasmid into SW480 and HCT116 cells. (A) Transfected cells were harvested at 72 h post-transfection and subjected to western blotting analysis for the evaluation of MTDH protein expression. *P<0.05 versus miR-NC. #P<0.05 versus miR-877 mimics + pcDNA3.1. (B and C) CCK-8 and colony formation assays were used to evaluate the proliferation and colony formation abilities of SW480 and HCT116 cells treated as above. *P<0.05 versus miR-NC. #P<0.05 versus miR-877 mimics + pcDNA3.1. (D) The apoptosis rate was measured using annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit. *P<0.05 versus miR-NC. #P<0.05 versus miR-877 mimics + pcDNA3.1. (E and F) Migration and invasion of SW480 and HCT116 cells co-transfected with miR-877 mimics and pc-MTDH or empty pcDNA3.1 were examined through Transwell migration and invasion assays. *P<0.05 versus miR-NC. #P<0.05 versus miR-877 mimics + pcDNA3.1.