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. 2019 May 2;9:6841. doi: 10.1038/s41598-019-43298-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of hepatic fibrosis by IC-2 sheets on the ninth day after transplantation. (A) Protocol of the animal experiment. Abbreviations: p.o., per os; Tx, transplantation. “p.o.” indicates oral administration of CCl_4. “Tx” indicates transplantation of cell sheets. (B) Micrographs of liver sections subjected to Azan staining (upper), Sirius red staining (middle), and immunohistochemistry for type I collagen (lower). MSC, mesenchymal stem cell. (C) The proportions of fibrotic areas among the groups (n = 7–9 except for n = 3 for CCl₄(−) group). CCl₄(−) indicates control mice without CCl₄ intoxication. (D) Hydroxyproline contents in the liver (n = 6 except for n = 3 for CCl₄(−) group). (E) Western blot of type I collagen in recipient liver tissues. The results are expressed as the mean ± S.E.M. Levels of significance: *P < 0.05; **P < 0.01 (one-way ANOVA followed by Games–Howell test).