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. 2019 Apr 2;11:2565–2578. doi: 10.2147/CMAR.S190332

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© 2019 Hao et al.

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NECTIN4 gene knockdown affects the proliferation of thyroid cancer cell lines.

Notes: (A–B) The relative expression of NECTIN4 (compared with the GAPDH gene) in TPC1 and KTC-1 via qRT-PCR and WB . Compared with corresponding Si-NC group, the expression of NECTIN4 in Si-RNA1 and Si-RNA2 group was lower. (C) Colony formation of NECTIN4 and si- NECTIN4 cells. (D–E) TPC1 and KTC-1 cell lines transfected with Si-RNA or Si-NC were cultured in 96-well plates for 1-4 days and using CCK-8 measured cell proliferation. Cell proliferation was significantly suppressed in thyroid cell transfected by Si-RNA1 and Si-RNA2.