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. 2019 May 2;9:6823. doi: 10.1038/s41598-019-43341-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cortical lesion depiction at the acute time point. A representative example of T2-weighted multi-slice MRI of a mouse 48 hours after dMCAO shows pronounced hyperintense regions in the right cortex covering a major part of the caudo-rostral extent. The image planes at the site of coagulation and the site where the hippocampal distortion is maximal are marked with a red frame. These two planes are used for further detailed analysis of the temporal development of the pathophysiological changes (cf. Fig. 3).