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. 2019 May 2;9:6823. doi: 10.1038/s41598-019-43341-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cortical volume changes after dMCAO. Quantitative analysis of the cortical tissue volume with hyperintensity (dark grey bar) and of the normal appearing tissue (light grey bar) is presented for the whole 12 weeks observation after dMCAO. While the hyperintensity is maximal at 48 hours with 38 ± 15% of cortical volume, it quickly decreases and reaches values of 0.6 ± 0.5% at week 12. Already visible at week 2 is the loss of cortical tissue, as the sum of light and dark grey bars are significantly less than the cortical volume of the contralateral hemisphere. This tissue loss further increases with time. Error bars represent standard deviation. All individual volume data of all stroke animals at all time points are compiled in the Supplement Table 1. Error bars are presented as SD.