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. 2019 Apr 9;6(2):ENEURO.0110-18.2019. doi: 10.1523/ENEURO.0110-18.2019

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Copyright © 2019 Gupta et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Adult-born GCs innervated by transplanted GABAergic interneurons develop significantly shorter distal dendrites. A, Bar graph of total dendritic lengths in transplant-innervated and non-innervated adult-born GCs in different experimental groups. All neurons were confirmed to be adult-born and RV labeled by immunostaining for mCherry expression. Data for non-innervated GCs is represented by purple bars and innervated GCs are shown in green bars. GCs with high levels of putative synaptic input from the transplanted GABAergic interneurons had significantly shorter dendrites than non-innervated adult-born GCs. Neuronal reconstructions of the GCs in different experimental groups are shown in Extended Data Figures 6-1, 6-2, 6-3, 6-4, 6-5. B, Schematic of the Sholl analysis paradigm for analysis of dendritic branching based on number of dendritic branches intersecting concentric spheres spaced at 10-µm intervals from the soma. C–H, Sholl analyses of dendritic arbors comparing transplant-innervated adult-born GCs (green lines) versus non-innervated GCs (purple lines). Shading represents SEM. C, In the naïve mice, innervated GCs showed reduced dendritic branching both proximally and distally, compared with non-innervated GCs. D, Grouped Sholl data showing significantly reduced dendritic arbors in innervated, adult-born GCs compared to non-innervated adult-born GCs in SE mice with transplants. While the innervated GCs formed similar patterns of proximal branching, they had significantly fewer distal branches, compared to non-innervated adult-born GCs. Significant differences found for radii at 150 μm from the soma (p = 0.03) and all radii between 170 and 290 μm from the soma (p < 0.01). E–H, Sholl data broken down by the birthdate post-SE of adult-born GCs. Graphs indicate that innervated adult-born GCs had significantly fewer distal dendrites, compared to non-innervated adult-born GCs. E, Innervated GCs born one week post-SE had significantly fewer intersections at radii between 200 and 270 μm from the soma, compared to non-innervated GCs (p < 0.05). F, Innervated GCs born two weeks post-SE had significantly reduced dendritic branching at radii 200–250 μm compared to the non-innervated GCs (p < 0.05). G, Innervated GCs born six weeks post-SE had significantly fewer dendritic branches at radii 150–270 μm compared to non-innervated GCs (p < 0.05). H, Innervated GCs born 12 weeks post-SE showed trend toward reduced branching compared to non-innervated GCs, but this trend did not reach significance. Asterisks indicate statistically significant differences between groups.