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. 2019 May 2;9:6821. doi: 10.1038/s41598-019-43092-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microbiota analyses of faecal samples. Summary of taxonomic identification by a three dimensional PCoA plot of LFD, HFD, ORL and L. sakei CJLS03 treatment groups using unweighted unifrac (a), showing percentage variation (beta diversity) for each of PC1, PC2, PC3 (b), taxonomic summary of the phylum level (c), the family level (d), and some of the major groups/genera within the Firmicutes (the Clostridium clusters IV and XIVa and Lactobacillus) and the genus Bifdobacterium (Actinobacteria) as measured by qPCR (e). ANOVA, Dunnett’s multiple comparisons test was used to distinguish the level of significance based on probability of 0.05 (*), 0.01 (**) and <0.001 (***) compared to the HFD group. Feeding groups: LFD: low-fat diet plus PBS; HFD: high-fat diet and PBS; ORL: high-fat diet plus orlistat; CJLS03 high-fat diet plus L. sakei CJLS03.