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MST measurement of full-length K-Ras4B and HVR-truncated K-Ras4B interaction with 5% PI(4,5)P2-doped DOPC lipid vesicles. A DOPC liposome doped with fluorescently labeled and unlabeled PI(4,5)P2 was titrated with a serial dilution of full-length K-Ras4B (1–188) G12V (black curve) or HVR truncated K-Ras4B (1–169) G12V (red curve) in a 2-fold dilution series.
