Figure 3.

Intracellular and extracellular levels of indol-3-ylmethyl glucosinolate–derived compounds in P. infestans-inoculated Arabidopsis leaves. Leaves of the Arabidopsis mutants pen3-1 (red bars), cad1-3 (blue bars), and pen2-1 (green bars), as well as their respective WTs Col-0 (black bars) and gl1 (gray bars) were drop-inoculated with a P. infestans zoospore suspension (5 × 10⁵ spores ml⁻¹). The inoculum and the leaf areas below the inoculation sites were collected separately after 24 h. Metabolite levels were determined by UPLC-ESI-QTOF-MS in methanolic extracts of inoculation sites (A) and in the inoculum (B). Data are derived from at least three independent experiments (n ≥ 15). Error bars, S.E. Significance analyses were performed using Mann-Whitney two-tailed U test (mutant versus respective WT; *, p < 0.05; **, p < 0.01; ***, p < 0.001). RA, raphanusamic acid.