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. 2019 Mar 4;294(17):6659–6669. doi: 10.1074/jbc.RA118.006375

Figure 3.

Figure 3.

RDE (II) inactivates serum IgE antibodies but not IgG. A and B, BALB/c were injected with pCADEST1–anti-HA IgG or anti-HA IgE and pCADEST1–anti-HA κ by hydrodynamic injection. One day later, the serum was obtained and treated with RDE (II) overnight ex vivo. Then, the specimens were incubated at 56 °C for 30 min. Anti-HA IgE and IgG levels were analyzed by quantitative ELISA. C and D, serum, which was obtained from naïve mice, was treated with RDE (II) overnight (12–20 h). Total IgE (C) and IgG (D) levels were analyzed by quantitative ELISA. Data are representative of two independent experiments and indicate the mean ± S.D. ***, p < 0.001 (Student's t test).