Figure 5.

Characteristics of RDE (II) for modulating IgE. A and B, RDE (II) was preincubated at 56 or 100 °C for 30 min. Purified IgE was treated with the RDE (II) overnight (12–20 h). The level of IgE was measured by quantitative ELISA (A), and they were also blotted and analyzed with HRP-conjugated goat anti-mouse IgE (B). C, purified IgE was treated with dialyzed RDE (II) overnight (12–20 h). They were blotted by Western blotting under nonreducing conditions. D, purified IgE were treated with RDE (II) for the indicated time. They were blotted under nonreducing conditions. Data are representative of two independent experiments and indicate the mean ± S.D. ***, p < 0.001 (one-way ANOVA). NS, no significance.