Figure 6.

Binding SAM stabilizes viperin in vivo. a, HEK 293T cells were transfected with viperin and 20 h post-transfection 50 mm cycloleucine, a SAM synthase inhibitor, was added to the medium. At various time points cells were harvested and viperin expression was analyzed by immunoblotting (IB). Depletion of cellular SAM by cycloleucine significantly decreases viperin half-life. b, HEK 293T cells were transfected with viperinΔ3C and 12 h post-transfection 50 nm sinefungin was added to the medium. At various time points cells were harvested and viperin expression was analyzed by immunoblotting. Sinefungin stabilizes viperinΔ3C compared with DMSO control. Lower panels, band intensities were quantified relative GAPDH and expressed as mean ± S.D. from three independent experiments.