Figure 3.

IL-32 transcriptional regulatory regions undergo DNA demethylation during 12 days of TNFα treatment. A, RT-qPCR analysis showed that the IL-32 mRNA level can be more efficiently activated via long-term TNFα treatment. Averages from three independent experiments are shown, and error bars represent standard deviation. B, Western blot results showed that the IL-32 protein level can be induced with 12 h and 12 days of TNFα treatment. C, locus-specific bisulfite sequencing results revealed that the promoter and CGI of the IL-32 gene are gradually demethylated during long-term TNFα treatment. Filled circles indicate methylated CpG sites, and open circles indicate unmethylated CpG sites. CpG site methylation percentages are shown.