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. 2019 Mar 1;294(17):6785–6795. doi: 10.1074/jbc.RA118.006255

Figure 4.

Figure 4.

IL-32 basal expression is up-regulated after long-term TNFα treatment and is accompanied by sustained hypomethylation at the promoter and CGI. A, locus-specific bisulfite sequencing data showed that the hypomethylation status of the IL-32 promoter and CGI can be maintained after 10 days of TNFα withdrawal. B, a time course experiment revealed that the IL-32 basal expression level is up-regulated after long-term TNFα treatment and TNFα withdrawal. Averages from three independent experiments are shown, and error bars represent standard deviation in the RT-qPCR results. d, day. C, Western blot results showed that cells treated long-term with TNFα display a higher basal protein expression level of IL-32. D, RT-qPCR results revealed that the up-regulation of IL-32 expression can be maintained for at least 30 days after TNFα withdrawal. Averages from three independent experiments are shown, and error bars represent standard deviation. E, bisulfite sequencing data revealed that cells subjected to 12 days of TNFα treatment maintained relatively low methylation levels at the promoter and CGI of the IL-32 gene even after 30 days of TNFα withdrawal.