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. 2019 Mar 1;294(17):6733–6750. doi: 10.1074/jbc.RA119.007671

Figure 2.

Figure 2.

NMK-T-057 induces reprogramming of EMT in BC cells. A, stress fiber assay of MDA-MB-231 cells treated with NMK-T-057 (0–5 μm) for 24 h in two different doses. Cells were stained with FITC-phalloidin to visualize actin stress fibers. Treatment resulted in disruption of actin filaments as compared with untreated controls. The image represents the best of the replicates (n = 3). Scale bars, 10 μm. B, Western blot images of vimentin, N-cadherin, E-cadherin, keratin-19, and TWIST in MDA-MB-231 cells treated with NMK-T-057 (0–5 μm). The blots represent the best of the replicates (n = 3). C, relative band intensities of vimentin (Vim), N-cadherin (N-cad), E-cadherin (E-cad), keratin-19 (K-19), and TWIST in MDA-MB-231cells treated with NMK-T-057 (0–5 μm). Data are expressed as mean ± S.E. (n = 3; *, p < 0.05 versus control (untreated cells). D, immunophenotyping with CD24-FITC and CD44-PE in MDA-MB-231 cells treated with 5 μm NMK-T-057. The data represent the best of the replicates (n = 3). Error bars represent S.E. in respective panels.