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. 2019 Mar 1;294(17):6733–6750. doi: 10.1074/jbc.RA119.007671

Figure 3.

Figure 3.

NMK-T-057 inhibits stemness in MDA-MB-231 cells. A, ALDEFLUOR assay of MDA-MB-231 cells treated with NMK-T-057 (0–5 μm) for 24 h. The data represent the best of the replicates (n = 3). B, phase-contrast microscopy images of primary (upper panel) and secondary (lower panel) mammospheres from MDA-MB-231 cells treated with NMK-T-057 (0–5 μm) for 24 h. Images were taken under 20× magnification and represent the best of the replicates (n = 3). Scale bar, 100 μm. C, graph showing the number of primary and secondary spheroids versus NMK-T-057 (0–5 μm). Data are expressed as mean ± S.E. (n = 3); *, p < 0.05 versus control (untreated cells). Error bars represent S.E. in respective panels.