Skip to main content
. 2019 Apr 26;57(5):e01794-18. doi: 10.1128/JCM.01794-18

TABLE 1.

Example pass-filter sequencing read numbers for multiple-HPV-type reaction mixtures containing 500-bp synthetic dsDNA control fragmentsa

10-copy HPV type 10K-copy HPV types No. of pass-filter sequencing reads for:
10-copy type 10K-copy HPV type
1 2 3 4 5
6 31, 44, 58, 68b, 81 13,960 27,989 36,273 10,510 13,310 16,258
11 32, 45, 59, 69, 82 7,885 15,595 28,385 22,554 15,510 13,559
16 35, 52, 62, 71, 83 23,270 16,563 12,738 24,447 5,200 4,032
18 39, 53, 64, 72, 84 20,277 19,995 3,075 2,092 2,904 1,442
31 44, 58, 68b, 81, 89 23,436 33,959 12,639 11,806 18,545 4,572
33 51, 61, 70, 82v, 91 20,886 28,692 8,566 28,812 10,283 10,923
35 52, 62, 71, 83, 97 16,371 18,776 23,378 5,593 2,797 25,275
39 53, 64, 72, 84, 114 2,512 8,991 2,880 4,860 4,157 15,011
45 11, 32, 59, 69, 82 11,567 15,791 15,259 24,270 16,179 7,767
51 13, 33, 61, 70, 82v 20,773 29,500 15,024 5,835 20,526 7,385
52 16, 35, 62, 71, 83 6,537 31,618 9,199 18,221 4,807 3,976
56 3, 30, 43, 68a, 76 6,443 9,707 6,570 15,466 23,385 14,178
58 6, 31, 44, 68b, 81 5,761 14,427 22,380 22,961 9,748 12,269
59 11, 32, 45, 69, 82 3,435 16,462 14,863 30,638 15,174 14,438
68a 3, 30, 43, 76, 87 19,633 9,999 4,956 18,635 16,509 8,347
68b 6, 31, 44, 81, 89 8,359 14,158 19,683 21,365 13,504 3,387
a

Each row represents a single test mixture, with the list of types in each mixture in the first two columns, and the number of reads detected for each of those types in columns 3 to 8. Only read numbers for target types are shown for readability. HPV68a (lineages A and B) and HPV68b (lineages C to F) are uniquely identifiable by TypeSeq and were tested individually.