FIG 1.

Linearity of cell-associated HIV (CAH) on dilutions of 8E5 cells. Washed 8E5 cultured cells containing a single copy of integrated HIV genome per cell were spiked into whole blood at 25, 50, 100, 200, 500, and 1,000 cells/ml blood. Seventy microliters of spiked blood was tested in triplicate by the CAP/CTM assay. The number of 8E5 cells was determined based upon the volume of sample tested. The reported CAP/CTM assay value was corrected for sample volume to obtain the number of CAH copies. Means and ranges of data values are shown for each triplicate measure. R² = 0.9502; y = 1.442 · x + 7.524. 1K, 1,000.