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Colistin broth-disk elution and EDTA colistin broth-disk elution methods. Colistin broth-disk elution (CBDE) with (A) and without (B) 1 mM EDTA (EDTA-CBDE). An mcr-1-producing Escherichia coli isolate (CDC AR Bank number 350) with a colistin MIC of 4 μg/ml based on the CBDE method. The colistin MIC is reduced to ≤1 μg/ml in the presence of EDTA, consistent with a positive EDTA-CBDE result and indicating plasmid-mediated colistin resistance. GC indicates growth control.
