TABLE 3.
Group 2 real-time PCR results using the laboratory-developed real-time assay for the detection of Dientamoeba fragilis following implementation of fluorescence thresholds or CT cutoff values
| Assay | Platform | No. positive | No. negative | % positive | CT range |
|---|---|---|---|---|---|
| EasyScreen (10) | Bio-Rad CFX96 | 0 | 200 | 0 | 0–0 |
| Laboratory-developed real-time PCR assay (9) | Cepheid SmartCycler II (CT cutoff, 37) | 5 | 195 | 2.5 | 32.00–45.16 |
| Roche LightCycler 480a | 4 | 196 | 2.0 | 39.59–45.00 | |
| Bio-Rad CFX96 (threshold, 12,000 RFU × 103) | 2 | 198 | 1.0 | 46.93–47.61 | |
| ABI 7500 (CT cutoff, 38) | 4 | 194 | 3.0 | 32.34–40.11 |
a
True-positive and false-positive results could not be distinguished via CT cutoff values or fluorescence thresholds on these platforms. (True positives were established via amplicon sequencing.)