FIG 1.

CD4 downregulation and GFP expression over time in CEM cells infected with HIV-1_AD8. (A) Flow cytometry scatter plots depicting CD4 and HIV-1_AD8 (GFP) expression over 12 to 48 h. The top panel shows HIV-1_AD8-infected CEM cells in the absence of T20. The middle and bottom panels show CEM cells treated with 10 μg/ml of T20 before or 4 h after spinoculation with HIV-1_AD8. The numbers represent the proportions of CD4^hi GFP^–, CD4^int GFP^lo, and CD4^lo GFP^hi cells, as indicated. (B) The proportions of CD4^int and CD4^lo GFP⁺ infected CEM cells over time. (C) CD4 expression in terms of median fluorescence intensity (MFI) on GFP⁺ infected CEM cells over time. The data in panels B and C were averaged from four independent experiments.