FIG 5.

ADCC against HIV-1_AD8-infected CEM cells sorted into GFP^–, GFP^lo, and GFP^hi populations. (A) Representative scatter plots showing elimination of the sorted target cells in the presence of effector NK cells with HIV-1⁺ plasma compared to NK cells with HIV-1^– plasma. Uninfected CEM cells stained with the cell proliferation dye eFluor 670 (eFluor 670⁺ GFP^–) were spiked into each sample at the end of the 5-h ADCC assay incubation to serve as a reference population to calculate percent ADCC of the sorted target cells. (B) ADCC mediated by HIV-1⁺ plasma (1:1,000; n = 14) against sorted GFP^– cells that remained GFP^– or became GFP⁺ after the 5-h assay incubation. (C) ADCC mediated by HIV-1⁺ plasma (1:1,000; n = 8) against sorted GFP^lo and GFP^hi cells. Statistical analyses between matched pairs were performed with a Wilcoxon signed-rank test (ns, not significant; ***, P < 0.001).