FIG 6.

Thermal stability of purified mutant empty capsids containing the lethal charged-to-neutral substitution H3141A and/or the viability-restoring, charge-compensating substitution N2019H relative to that of the wt empty capsid control. (A) Percentage of intact capsids remaining after different incubation times at 42°C in a representative experiment. Open circles, wt capsids; filled triangles, mutant H3141A; filled inverted triangles, mutant N2019H; filled squares, mutant H3141A/N2019H. Data were fitted to exponential decay processes, and dissociation rate constants were obtained. The gray curve was obtained using the control wt capsids. (B) Ratios between the rate constant obtained for each mutant and the rate constant obtained for the wt control. Values are averages and standard deviations obtained from 3 (N2019H) or 4 (all other variants) independent experiments.