FIG 9.

V1 domain length serves as a mechanism of escape from some V3-glycan broadly neutralizing antibodies. NL-VA40774 (red) and NL-VA40774(AD8V1) (blue) were tested for their capacity to be neutralized by V3-glycan broadly neutralizing antibodies (PGT121, PGT128, 10-1074). Exchanging the long V1 domain of NL-VA40774 with the short V1 domain of NL-AD8 rendered the virus at least 500-fold more susceptible to neutralization by PGT121 and 10-fold more susceptible to 10-1074. Exchange of the long V1 domain with a short V1 domain had no effect on neutralization by PGT128.